A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.
ENZYME entry: EC 1.13.12.7
| Accepted Name |
|---|
| Firefly luciferase.
|
| Alternative Name(s) |
|---|
| Luciferase. |
| Photinus pyralis luciferase. |
| Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing). |
| Reaction catalysed |
|---|
| D-firefly luciferin + O(2) + ATP <=> firefly oxyluciferin + CO(2) + AMP + diphosphate + light |
| Comment(s) |
|---|
- The enzyme, which is found in fireflies (Lampyridae), is responsible
for their biolouminescence.
- The reaction begins with the formation of an acid anhydride between
the carboxylic group of D-firefly luciferin and AMP, with the release
of diphosphate.
- An oxygenation follows, with release of the AMP group and formation
of a very short-lived peroxide that cyclizes into a dioxetanone
structure, which collapses, releasing a CO(2) molecule.
- The spontaneous breakdown of the dioxetanone (rather than the
hydrolysis of the adenylate) releases the energy (about 50 kcal/mole)
that is necessary to generate the excited state of oxyluciferin.
- The excited luciferin then emits a photon, returning to its ground
state.
- The enzyme has a secondary acyl-CoA ligase activity when acting on
L-firefly luciferin.
|
| Cross-references |
|---|
| PROSITE | PDOC00427 |
| BRENDA | 1.13.12.7 |
| EC2PDB | 1.13.12.7 |
| ExplorEnz | 1.13.12.7 |
| PRIAM enzyme-specific profiles | 1.13.12.7 |
| KEGG Ligand Database for Enzyme Nomenclature | 1.13.12.7 |
| IUBMB Enzyme Nomenclature | 1.13.12.7 |
| IntEnz | 1.13.12.7 |
| MEDLINE | Find literature relating to 1.13.12.7 |
| MetaCyc | 1.13.12.7 |
| UniProtKB/Swiss-Prot |
|
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