ID 1.14.13.234 DE 12-dehydrotetracycline 5-monooxygenase. CA 5a,11a-dehydrotetracycline + H(+) + NADPH + O2 = 5a,11a- CA dehydrooxytetracycline + H2O + NADP(+). CC -!- The enzyme, characterized from the bacterium Streptomyces rimosus, CC is bifunctional, catalyzing two successive monooxygenation reactions. CC -!- It starts by catalyzing the stereospecific hydroxylation of CC anhydrotetracycline at C(6) (EC 1.14.13.38). CC -!- If the released product is captured by EC 1.3.98.4 it is reduced to CC tetracycline. CC -!- However, if the released product is recaptured by OxyS, it performs CC an additional hydroxylation at C(5), producing 5a,11a- CC dehydrooxytetracycline, which, following the action of EC 1.3.98.4, CC becomes oxytetracycline. DR L8EUQ6, OXYS_STRR1 ; //