ID 1.14.13.242 DE 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase. AN 3-hydroxy-2-methylpyridinecarboxylate dioxygenase. AN methylhydroxypyridine carboxylate dioxygenase. AN methylhydroxypyridinecarboxylate oxidase. AN MHPCO. CA (1) 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 = CA 2-(acetamidomethylene)succinate + NAD(+). CA (2) 3-hydroxy-2-methylpyridine-5-carboxylate + NADPH + O2 = CA 2-(acetamidomethylene)succinate + NADP(+). CC -!- The enzyme, characterized from the bacteria Pseudomonas sp. MA-1 and CC Mesorhizobium loti, participates in the degradation of pyridoxine CC (vitamin B6). CC -!- Although the enzyme was initially thought to be a dioxygenase, CC oxygen-tracer experiments have shown that it is a monooxygnase, CC incorporating only one oxygen atom from molecular oxygen. CC -!- The second oxygen atom that is incorporated into the product CC originates from a water molecule, which is regenerated during the CC reaction and thus does not show up in the reaction equation. CC -!- Formerly EC 1.14.12.4. //