ID   1.14.13.243
DE   toluene 2-monooxygenase.
AN   toluene ortho-monooxygenase.
CA   (1) toluene + NADH + O2 + H(+) = 2-hydroxytoluene + NAD(+) + H2O.
CA   (2) 2-hydroxytoluene + NADH + O2 + H(+) = 3-methylcatechol + NAD(+) +
CA   H2O.
CC   -!- The enzyme, characterized from the bacterium Burkholderia cepacia,
CC       belongs to a class of nonheme, oxygen-dependent diiron enzymes.
CC   -!- It contains a hydroxylase component with two binuclear iron centers,
CC       an NADH-oxidoreductase component containing FAD and a [2Fe-2S] iron-
CC       sulfur cluster, and a third component involved in electron transfer
CC       between the hydroxylase and the reductase.
CC   -!- The enzyme dihydroxylates its substrate in two sequential
CC       hydroxylations, initially forming 2-methylphenol, which is
CC       hydroxylated to 3-methylcatechol.
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