ID 1.14.14.13 DE 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] DE monooxygenase. CA 4-(gamma-L-glutamylamino)butanoy-[BtrI ACP] + FMNH2 + O2 = 4-(gamma-L- CA glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI ACP] + FMN + H(+) + H2O. CC -!- Catalyzes a step in the biosynthesis of the side chain of the CC aminoglycoside antibiotics of the butirosin family. CC -!- Forms a complex with a dedicated NAD(P)H:FMN oxidoreductase. CC -!- The enzyme is not able to hydroxylate free substrates, activation by CC the acyl-carrier protein is mandatory. CC -!- Octanoyl-S-[BtrI acyl-carrier protein] is also accepted. DR Q4H4E5, BTRO_NIACI ; Q4H4E3, BTRV_NIACI ; //