ID   1.14.14.172
DE   3,5,6-trichloropyridin-2-ol monooxygenase.
CA   (1) 3,5,6-trichloropyridin-2-ol + FADH2 + O2 = 3,6-dichloropyridine-2,5-
CA   dione + chloride + FAD + 2 H(+) + H2O.
CA   (2) 3,6-dichloropyridine-2,5-diol + FADH2 + O2 = 6-chloro-3-
CA   hydroxypyridine-2,5-dione + chloride + FAD + 2 H(+) + H2O.
CA   (3) 6-chloropyridine-2,3,5-triol + FADH2 + O2 = 3,6-dihydroxypyridine-
CA   2,5-dione + chloride + FAD + 3 H(+) + H2O.
CC   -!- The enzyme, characterized from a number of bacterial species,
CC       participates in the degradation of 3,5,6-trichloropyridin-2-ol (TCP),
CC       a metabolite of the common organophosphorus insecticide chlorpyrifos.
CC   -!- The enzyme is a multifunctional flavin-dependent monooxygenase that
CC       displaces three chlorine atoms by attacking three different positions
CC       in the substrate.
CC   -!- Each reaction catalyzed by the enzyme displaces a single chlorine and
CC       results in formation of a dione, which must be reduced by FADH2
CC       before the monooxygenase could catalyze the next step.
CC   -!- The large amount of FADH2 that is required is generated by a
CC       dedicated flavin reductase (TcpX). cf. EC 1.14.14.173.
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