ID 1.14.14.173 DE 2,4,6-trichlorophenol monooxygenase. CA 2,4,6-trichlorophenol + FADH2 + O2 = 2-chloro-6-hydroxy-1,4-benzoquinone CA + 2 chloride + FAD + 3 H(+). CC -!- The enzyme, characterized from Cupriavidus pinatubonensis, CC participates in the degradation of 2,4,6-trichlorophenol, a compound CC that has been used for decades as a wood preservative. CC -!- The enzyme is a multifunctional flavin-dependent monooxygenase that CC catalyzes two different reactions to displace two chlorine atoms, CC a monooxygenase reaction followed by a hydrolysis reaction that takes CC advantage of the reactivity of the product of the first reaction, CC 2,6-dichloro-1,4-benzoquinone. CC -!- The large amount of FADH2 that is required is generated by a CC dedicated flavin reductase (TcpB). cf. EC 1.14.14.172. DR Q471I2, TCPA_CUPPJ ; //