ID 1.14.14.178 DE steroid 22S-hydroxylase. AN CYP90B1. AN steroid C-22 hydroxylase. CA (1) a C27-steroid + O2 + reduced [NADPH--hemoprotein reductase] = CA a (22S)-22-hydroxy C27-steroid + H(+) + H2O + oxidized [NADPH- CA -hemoprotein reductase]. CA (2) a C28-steroid + O2 + reduced [NADPH--hemoprotein reductase] = CA a (22S)-22-hydroxy C28-steroid + H(+) + H2O + oxidized [NADPH- CA -hemoprotein reductase]. CA (3) a C29-steroid + O2 + reduced [NADPH--hemoprotein reductase] = CA a (22S)-22-hydroxy C29-steroid + H(+) + H2O + oxidized [NADPH- CA -hemoprotein reductase]. CC -!- This plant cytochrome P450 (heme thiolate) enzyme participates in the CC biosynthesis of brassinosteroids. CC -!- While in vivo substrates include C28-steroids such as campestanol, CC campesterol, and 6-oxocampestanol, the enzyme is able to catalyze the CC C-22 hydroxylation of a variety of C27, C28 and C29 steroids. DR O64989, C90B1_ARATH; I1H7R8, C90B1_BRADI; B8AJL3, C90B2_ORYSI; DR Q5CCK3, C90B2_ORYSJ; //