ID   1.14.14.39
DE   isoleucine N-monooxygenase.
AN   CYP79D3.
AN   CYP79D4.
CA   L-isoleucine + 2 O2 + 2 reduced [NADPH--hemoprotein reductase] = (1E,2S)-
CA   2-methylbutanal oxime + CO2 + 2 H(+) + 3 H2O + 2 oxidized [NADPH-
CA   -hemoprotein reductase].
CC   -!- This enzyme, found in plants, catalyzes two successive
CC       N-hydroxylations of L-isoleucine, the committed step in the
CC       biosynthesis of the cyanogenic glucoside lotaustralin.
CC   -!- The product of the two hydroxylations, N,N-dihydroxy-L-isoleucine,
CC       is labile and undergoes dehydration followed by decarboxylation,
CC       producing the oxime.
CC   -!- It is still not known whether the decarboxylation is spontaneous or
CC       catalyzed by the enzyme.
CC   -!- The enzyme can also accept L-valine, but with a lower activity. cf.
CC       EC 1.14.14.38.
CC   -!- Formerly EC 1.14.13.117.
DR   Q6J541, C79D3_LOTJA;  Q6J540, C79D4_LOTJA;
//