ID   1.14.14.43
DE   (methylsulfanyl)alkanaldoxime N-monooxygenase.
AN   (methylthio)alkanaldoxime N-monooxygenase.
AN   CYP83A1.
CA   an (E)-omega-(methylsulfanyl)-alkanal oxime + glutathione + O2 + reduced
CA   [NADPH--hemoprotein reductase] = an S-[(1E)-1-(hydroxyimino)-omega-
CA   (methylsulfanyl)alkyl]-L-glutathione + H(+) + 2 H2O + oxidized [NADPH-
CA   -hemoprotein reductase].
CC   -!- Involved in the biosynthesis of glucosinolates in plants.
CC   -!- Catalyzes an N-hydroxylation of the E isomer of
CC       n-(methylsulfanyl)aldoximes, forming an aci-nitro intermediate that
CC       reacts non-enzymically with glutathione to produce an N-alkyl-
CC       thiohydroximate adduct, the committed precursor of glucosinolates.
CC   -!- In the absence of a thiol compound, the enzyme is suicidal, probably
CC       due to interaction of the reactive aci-nitro intermediate with active
CC       site residues.
DR   P48421, C83A1_ARATH;
//