ID   1.14.14.45
DE   aromatic aldoxime N-monooxygenase.
AN   CYP83B1.
CA   (1) (E)-(indol-3-yl)acetaldehyde oxime + glutathione + O2 + reduced
CA   [NADPH--hemoprotein reductase] = (E)-1-(glutathione-S-yl)-2-(1H-indol-3-
CA   yl)acetohydroximate + H(+) + 2 H2O + oxidized [NADPH--hemoprotein
CA   reductase].
CA   (2) (E)-phenylacetaldehyde oxime + glutathione + O2 + reduced [NADPH-
CA   -hemoprotein reductase] = (Z)-1-(glutathione-S-yl)-2-
CA   phenylacetohydroximate + H(+) + 2 H2O + oxidized [NADPH--hemoprotein
CA   reductase].
CC   -!- Involved in the biosynthesis of glucosinolates in plants.
CC   -!- The enzyme catalyzes the N-hydroxylation of aromatic aldoximes
CC       derived from L-tryptophan and L-phenylalanine, forming an aci-nitro
CC       intermediate that reacts non-enzymically with glutathione to produce
CC       an N-alkyl-thiohydroximate adduct, the committed precursor of
CC       glucosinolates.
CC   -!- In the absence of glutathione, the enzyme is suicidal, probably due
CC       to interaction of the reactive aci-nitro compound with catalytic
CC       residues in the active site.
DR   O65782, C83B1_ARATH;
//