ID   1.14.15.26
DE   toluene methyl-monooxygenase.
CA   (1) toluene + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = benzyl
CA   alcohol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O.
CA   (2) p-xylene + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) =
CA   4-methylbenzyl alcohol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O.
CA   (3) m-xylene + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) =
CA   3-methylbenzyl alcohol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O.
CC   -!- The enzyme, characterized from several Pseudomonas strains, catalyzes
CC       the first step in the degradation of toluenes and xylenes.
CC   -!- It has a broad substrate specificity and is also active with
CC       substituted compounds, such as chlorotoluenes.
CC   -!- The electrons are provided by a reductase (EC 1.18.1.3) that
CC       transfers electrons from NADH via FAD and an [2Fe-2S] cluster.
CC   -!- The enzyme can also act on its products, producing gem-diols that
CC       spontaneously dehydrate to form aldehydes.
DR   P21395, XYLM_PSEPU ;
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