ID   1.14.15.28
DE   cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate
DE   forming].
CA   cholest-4-en-3-one + 5 H(+) + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] =
CA   (25R)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-
CA   [ferredoxin].
CC   -!- This cytochrome P450 (heme-thiolate) enzyme, found in several
CC       bacterial pathogens, is involved in degradation of the host
CC       cholesterol.
CC   -!- It catalyzes the hydroxylation of the C-26 carbon, followed by
CC       oxidation of the alcohol to the carboxylic acid via the aldehyde
CC       intermediate, initiating the degradation of the alkyl side-chain of
CC       cholesterol.
CC   -!- The products are exclusively in the (25R) conformation.
CC   -!- The enzyme also accepts cholesterol as a substrate. cf. 1.14.15.29.
CC   -!- The enzyme can receive electrons from ferredoxin reductase in vitro,
CC       its natural electron donor is not known yet.
CC   -!- Formerly EC 1.14.13.221.
DR   P0A517, CP124_MYCBO;  P9WPP2, CP124_MYCTO;  P9WPP3, CP124_MYCTU;
DR   A0R4Q6, CP142_MYCS2;  P9WPL4, CP142_MYCTO;  P9WPL5, CP142_MYCTU;
//