ID   1.14.15.29
DE   cholest-4-en-3-one 26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate
DE   forming].
AN   cholest-4-en-3-one 26-monooxygenase.
AN   cholest-4-en-3-one 27-monooxygenase.
AN   CYP125.
AN   CYP125A1.
CA   cholest-4-en-3-one + 5 H(+) + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] =
CA   (25S)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-
CA   [ferredoxin].
CC   -!- A cytochrome P450 (heme-thiolate) protein found in several bacterial
CC       pathogens.
CC   -!- The enzyme is involved in degradation of the host's cholesterol.
CC   -!- It catalyzes the hydroxylation of the C-26 carbon, followed by
CC       oxidation of the alcohol to the carboxylic acid via the aldehyde
CC       intermediate, initiating the degradation of the alkyl side-chain of
CC       cholesterol.
CC   -!- The products are exclusively in the (25S) conformation.
CC   -!- The enzyme also accepts cholesterol as a substrate. cf.
CC       EC 1.14.15.28.
CC   -!- Formerly EC 1.14.13.141.
DR   P63710, CP125_MYCBO;  A0R4Y3, CP125_MYCS2;  P9WPP0, CP125_MYCTO;
DR   P9WPP1, CP125_MYCTU;  Q0S7M1, CP125_RHOJR;
//