ID 1.14.19.21 DE cholesterol 7-desaturase. CA (1) cholesterol + H(+) + NADPH + O2 = 7-dehydrocholesterol + 2 H2O + CA NADP(+). CA (2) cholesterol + H(+) + NADH + O2 = 7-dehydrocholesterol + 2 H2O + CA NAD(+). CC -!- The enzyme, characterized from several organisms including the worm CC Caenorhabditis elegans, the fly Drosophila melanogaster, and the CC ciliate Tetrahymena thermophila, is a Rieske oxygenase. CC -!- In insects it participates in the the biosythesis of ecdysteroid CC hormones. CC -!- The electrons are transferred from NAD(P)H via an electron transfer CC chain likely to include ferredoxin reductase and ferredoxin. CC -!- The enzyme differs from regular desaturases, such as EC 1.14.19.20, CC which are cytochrome b5-dependent and contain the three His-boxes CC that are typical to most desaturases. DR Q17938, DAF36_CAEEL; F7J186, NVD1_CIOIN ; F7J187, NVD2_CIOIN ; DR Q1JUZ2, NVD_BOMMO ; Q6DHJ3, NVD_DANRE ; Q1JUZ1, NVD_DROME ; DR F7J188, NVD_HEMPU ; F7J184, NVD_XENLA ; //