ID   1.14.99.38
DE   cholesterol 25-monooxygenase.
AN   cholesterol 25-hydroxylase.
CA   AH2 + cholesterol + O2 = 25-hydroxycholesterol + A + H2O.
CC   -!- Unlike most other sterol hydroxylases, this enzyme is not a
CC       cytochrome P450.
CC   -!- Instead, it uses diiron cofactors to catalyze the hydroxylation of
CC       hydrophobic substrates.
CC   -!- The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to
CC       the enzyme through interactions with clustered histidine or glutamate
CC       residues.
CC   -!- In cell cultures, this enzyme down-regulates cholesterol synthesis
CC       and the processing of sterol regulatory element binding proteins
CC       (SREBPs). cf. EC 1.17.99.10.
DR   Q5PRC0, CH25H_DANRE;  O95992, CH25H_HUMAN;  Q9Z0F5, CH25H_MOUSE;
DR   Q4G1G8, CH25H_PIG  ;  Q4QQV7, CH25H_RAT  ;
//