ID 1.14.99.67 DE alpha-N-dichloroacetyl-p-aminophenylserinol N-oxygenase. CA AH2 + alpha-N-dichloroacetyl-p-aminophenylserinol + 2 O2 = A + CA chloramphenicol + 2 H2O. CC -!- The enzyme, isolated from the bacterium Streptomyces venezuelae, CC is involved in the biosynthesis of the antibiotic chloramphenicol. CC -!- It contains a carboxylate-bridged binuclear non-heme iron cluster. CC -!- The components of the native electron chain have not been identified, CC although the immediate donor is likely to be an iron-sulfur protein. CC -!- The reaction mechanism involves formation of an extremely stable CC peroxo intermediate that catalyzes three individual two-electron CC oxidations via a hydroxylamine and a nitroso intermediates without CC releasing the intermediates. cf. EC 1.14.99.68. DR F2RB83, CMLI_STRVP ; //