ID 1.14.99.69 DE tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase. AN tRNA 2-(methylsulfanyl)-N(6)-dimethylallyladenosine(37) hydroxylase. AN tRNA 2-methylthio-N(6)-isopentenyl adenosine(37) hydroxylase. CA 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + AH2 + O2 = A + CA H2O + N(6)-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2- CA (methylsulfanyl)adenosine(37) in tRNA. CC -!- The enzyme, found only within a small subset of facultative anaerobic CC bacteria, belongs to the nonheme diiron family. CC -!- The enzyme from Salmonella typhimurium was shown to catalyze a CC stereoselective (E) hydroxylation at the terminal C(4)-position of CC the prenyl group. DR Q88KV1, MIAE_PSEPK ; Q08015, MIAE_SALTY ; //