ID 1.17.99.3 DE 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase. AN 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate 24-hydroxylase. AN 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA oxidase. AN THCA-CoA oxidase. AN THC-CoA oxidase. AN trihydroxycoprostanoyl-CoA oxidase. CA (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + A + CA H2O = (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26- CA oyl-CoA + AH2. CC -!- Requires ATP. CC -!- The reaction in mammals possibly involves dehydrogenation to give a CC 24(25)-double bond followed by hydration. CC -!- However, in amphibians such as the Oriental fire-bellied toad CC (Bombina orientalis), it is probable that the product is formed via CC direct hydroxylation of the saturated side chain of (25R)- CC 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate and not via CC hydration of a 24(25) double bond. CC -!- In microsomes, the free acid is preferred to the coenzyme A ester, CC whereas in mitochondria, the coenzyme A ester is preferred to the CC free-acid form of the substrate. DR Q99424, ACOX2_HUMAN; Q9QXD1, ACOX2_MOUSE; O02767, ACOX2_RABIT; DR P97562, ACOX2_RAT ; //