ID   1.20.4.1
DE   arsenate reductase (glutathione/glutaredoxin).
AN   arsenate reductase (glutaredoxin).
CA   [glutaredoxin]-dithiol + arsenate + glutathione + H(+) = arsenite +
CA   glutathionyl-S-S-[glutaredoxin] + H2O.
CC   -!- The enzyme is part of a system for detoxifying arsenate.
CC   -!- The substrate binds to a catalytic cysteine residue, forming a
CC       covalent thiolate--As(V) intermediate.
CC   -!- A tertiary intermediate is then formed between the arsenic,
CC       the enzyme's cysteine, and a glutathione cysteine.
CC   -!- This intermediate is reduced by glutaredoxin, which forms a dithiol
CC       with the glutathione, leading to the dissociation of arsenite.
CC   -!- Thus reduction of As(V) is mediated by three cysteine residues: one
CC       in ArsC, one in glutathione, and one in glutaredoxin.
CC   -!- Although the arsenite formed is more toxic than arsenate, it can be
CC       extruded from some bacteria by EC 7.3.2.7; in other organisms,
CC       arsenite can be methylated by EC 2.1.1.137 in a pathway that produces
CC       non-toxic organoarsenical compounds; cf. EC 1.20.4.4.
CC   -!- Formerly EC 1.97.1.5.
DR   Q336V5, ACR21_ORYSJ;  Q10SX6, ACR22_ORYSJ;  P08692, ARSC1_ECOLX;
DR   P52147, ARSC2_ECOLX;  P0CF87, ARSCL_ECOLI;  O50595, ARSC_ACIMA ;
DR   P0AB96, ARSC_ECOLI ;  P44589, ARSC_HAEIN ;  P95354, ARSC_NEIGO ;
DR   P63621, ARSC_NEIMA ;  P63622, ARSC_NEIMB ;  Q92R44, ARSC_RHIME ;
DR   P0AB97, ARSC_SHIFL ;  P74313, ARSC_SYNY3 ;  P74984, ARSC_YEREN ;
DR   Q6ZEM6, ARSI1_SYNY3;  Q6YRW7, ARSI2_SYNY3;  Q8GY31, CDC25_ARATH;
DR   Q8RUD6, HARC1_ARATH;
//