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ENZYME entry: EC 1.4.1.13
Accepted Name |
glutamate synthase (NADPH).
|
Alternative Name(s) |
glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase. |
glutamate synthetase (NADP). |
glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP). |
glutamine-ketoglutaric aminotransferase. |
GOGAT. |
L-glutamate synthase. |
L-glutamate synthetase. |
NADPH-dependent glutamate synthase. |
NADPH-glutamate synthase. |
Reaction catalysed |
2 L-glutamate + NADP(+) <=> 2-oxoglutarate + H(+) + L-glutamine + NADPH |
Comment(s) |
- The reaction takes place in the opposite direction.
- The protein is composed of two subunits, alpha and beta.
- The alpha subunit is composed of two domains, one hydrolyzing
L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2), the other
combining the produced NH3 with 2-oxoglutarate to produce a second
molecule of L-glutamate (cf. EC 1.4.1.4).
- The beta subunit transfers electrons from the cosubstrate.
- The NH3 is channeled through a 31 A channel in the active protein.
- In the absence of the beta subunit, coupling between the two domains
of the alpha subunit is compromised and some ammonium can be
produced.
- In the intact alphabeta complex, ammonia production only takes place
as part of the overall reaction.
- Formerly EC 2.6.1.53.
|
Cross-references |
BRENDA | 1.4.1.13 |
EC2PDB | 1.4.1.13 |
ExplorEnz | 1.4.1.13 |
PRIAM enzyme-specific profiles | 1.4.1.13 |
KEGG Ligand Database for Enzyme Nomenclature | 1.4.1.13 |
IUBMB Enzyme Nomenclature | 1.4.1.13 |
IntEnz | 1.4.1.13 |
MEDLINE | Find literature relating to 1.4.1.13 |
MetaCyc | 1.4.1.13 |
Rhea expert-curated reactions | 1.4.1.13 |
UniProtKB/Swiss-Prot |
O29309, AGLUS_ARCFU; | Q3Z7F6, AGLUS_DEHM1; | Q58746, AGLUS_METJA; |
Q9WYM8, AGLUS_THEMA; | B9KBQ2, AGLUS_THENN; | B1L993, AGLUS_THESQ; |
P39812, GLTA_BACSU; | Q05755, GLTB_AZOBR; | O34399, GLTB_BACSU; |
P09831, GLTB_ECOLI; | E1V8I1, GLTB_HALED; | P96218, GLTB_MYCTU; |
Q05756, GLTD_AZOBR; | O08340, GLTD_CERSP; | P09832, GLTD_ECOLI; |
E1V8I0, GLTD_HALED; | P9WN18, GLTD_MYCTO; | P9WN19, GLTD_MYCTU; |
|
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ENZYME /
UniProtKB/Swiss-Prot entries corresponding to 1.4.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.4.-.-
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