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ENZYME

ENZYME entry: EC 1.4.1.13

PURL: https://purl.expasy.org/enzyme/EC/1.4.1.13
Accepted Name
glutamate synthase (NADPH)
Alternative Name(s)
glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase
glutamate synthetase (NADP)
glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP)
glutamine-ketoglutaric aminotransferase
GOGAT
L-glutamate synthase
L-glutamate synthetase
NADPH-dependent glutamate synthase
NADPH-glutamate synthase
Reaction catalysed
2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH + H(+)
Comment(s)
  • The reaction takes place in the opposite direction.
  • The protein is composed of two subunits, alpha and beta.
  • The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4).
  • The beta subunit transfers electrons from the cosubstrate.
  • The NH3 is channeled through a 31 A channel in the active protein.
  • In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced.
  • In the intact alphabeta complex, ammonia production only takes place as part of the overall reaction.
  • Formerly EC 2.6.1.53.
Cross-references
BRENDA1.4.1.13
EC2PDB1.4.1.13
ExplorEnz1.4.1.13
KEGG Ligand Database for Enzyme Nomenclature1.4.1.13
IUBMB Enzyme Nomenclature1.4.1.13
MEDLINEFind literature relating to 1.4.1.13
MetaCyc1.4.1.13
Rhea expert-curated reactions1.4.1.13
UniProtKB/Swiss-Prot
O29309, AGLUS_ARCFUQ3Z7F6, AGLUS_DEHM1Q58746, AGLUS_METJA
Q9WYM8, AGLUS_THEMAB9KBQ2, AGLUS_THENNB1L993, AGLUS_THESQ
P39812, GLTA_BACSUQ05755, GLTB_AZOBRO34399, GLTB_BACSU
P09831, GLTB_ECOLIE1V8I1, GLTB_HALEDP96218, GLTB_MYCTU
Q05756, GLTD_AZOBRO08340, GLTD_CERSPP09832, GLTD_ECOLI
E1V8I0, GLTD_HALEDP9WN18, GLTD_MYCTOP9WN19, GLTD_MYCTU

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.4.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.4.-.-
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