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ENZYME entry: EC 1.4.1.13

Accepted Name
Glutamate synthase (NADPH).
Alternative Name(s)
Glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase.
Glutamate synthetase (NADP).
Glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP).
Glutamine-ketoglutaric aminotransferase.
GOGAT.
L-glutamate synthase.
L-glutamate synthetase.
NADPH-dependent glutamate synthase.
NADPH-glutamate synthase.
Reaction catalysed
2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
Cofactor(s)
FAD; FMN; Iron-sulfur.
Comment(s)
  • The reaction takes place in the opposite direction.
  • The protein is composed of two subunits, alpha and beta.
  • The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH(3) and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH(3) with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4).
  • The beta subunit transfers electrons from the cosubstrate.
  • The NH(3) is channeled through a 31 A channel in the active protein.
  • In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced.
  • In the intact alpha-beta complex, ammonia production only takes place as part of the overall reaction.
  • Formerly EC 2.6.1.53.
Cross-references
PROSITEPDOC00406
BRENDA1.4.1.13
EC2PDB1.4.1.13
ExplorEnz1.4.1.13
PRIAM enzyme-specific profiles1.4.1.13
KEGG Ligand Database for Enzyme Nomenclature1.4.1.13
IUBMB Enzyme Nomenclature1.4.1.13
IntEnz1.4.1.13
MEDLINEFind literature relating to 1.4.1.13
MetaCyc1.4.1.13
UniProtKB/Swiss-Prot
O29309, AGLUS_ARCFU;  Q3Z7F6, AGLUS_DEHM1;  Q58746, AGLUS_METJA;  
Q9WYM8, AGLUS_THEMA;  B9KBQ2, AGLUS_THENN;  B1L993, AGLUS_THESQ;  
Q9C102, GLT1_SCHPO;  P39812, GLTA_BACSU;  Q05755, GLTB_AZOBR;  
O34399, GLTB_BACSU;  P09831, GLTB_ECOLI;  E1V8I1, GLTB_HALED;  
P96218, GLTB_MYCTU;  Q05756, GLTD_AZOBR;  P09832, GLTD_ECOLI;  
E1V8I0, GLTD_HALED;  P9WN18, GLTD_MYCTO;  P9WN19, GLTD_MYCTU;  
O08340, GLTD_RHOSH;  

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