ID 1.5.3.24 DE sarcosine oxidase (5,10-methylenetetrahydrofolate-forming). CA (6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10-methylene- CA 5,6,7,8-tetrahydrofolate + glycine + H2O2. CC -!- The enzyme, found in some bacterial species, is composed of four CC different subunits and two active sites connected by a large CC 'reaction chamber'. CC -!- An imine intermediate is transferred between the sites, eliminating CC the production of toxic formaldehyde. CC -!- The enzyme contains three cofactors: non-covalently bound FAD and CC NAD(+), and FMN that is covalently bound to a histidine residue. CC -!- In the absence of folate the enzyme catalyzes the reaction of CC EC 1.5.3.1. //