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ENZYME entry: EC 2.1.1.200

Accepted Name
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.
Alternative Name(s)
TrMet(Xm32).
Reaction catalysed
  • cytidine(32) in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine
  • S-adenosyl-L-methionine + uridine(32) in tRNA <=> 2'-O-methyluridine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine
Comment(s)
  • In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine(32) in tRNA.
  • No methylation of cytosine(34) in tRNA(Leu)(CAA).
  • In vitro the enzyme 2'-O-methylates cytidine(34) of tRNA(Ser1) and uridine(32) of tRNA(Gln2).
Cross-references
BRENDA2.1.1.200
EC2PDB2.1.1.200
ExplorEnz2.1.1.200
PRIAM enzyme-specific profiles2.1.1.200
KEGG Ligand Database for Enzyme Nomenclature2.1.1.200
IUBMB Enzyme Nomenclature2.1.1.200
IntEnz2.1.1.200
MEDLINEFind literature relating to 2.1.1.200
MetaCyc2.1.1.200
Rhea expert-curated reactions2.1.1.200
UniProtKB/Swiss-Prot
A8AD50, TRMJ_CITK8;  A7MGX5, TRMJ_CROS8;  Q8XA83, TRMJ_ECO57;  
A1AE69, TRMJ_ECOK1;  Q0TEV3, TRMJ_ECOL5;  P0AE02, TRMJ_ECOL6;  
P0AE01, TRMJ_ECOLI;  Q1R8K0, TRMJ_ECOUT;  A6TCF3, TRMJ_KLEP7;  
Q6D257, TRMJ_PECAS;  Q7N222, TRMJ_PHOLL;  A0A0H2ZF87, TRMJ_PSEAB;  
Q57LG7, TRMJ_SALCH;  Q5PNG3, TRMJ_SALPA;  P66975, TRMJ_SALTI;  
P66974, TRMJ_SALTY;  Q31XV8, TRMJ_SHIBS;  Q32D32, TRMJ_SHIDS;  
Q0T1Y7, TRMJ_SHIF8;  P0AE03, TRMJ_SHIFL;  Q3YZ20, TRMJ_SHISS;  
Q2NS29, TRMJ_SODGM;  A1JKQ0, TRMJ_YERE8;  Q1C5G8, TRMJ_YERPA;  
Q7CJN8, TRMJ_YERPE;  Q1CKB1, TRMJ_YERPN;  A4TMV6, TRMJ_YERPP;  
Q667X9, TRMJ_YERPS;  

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