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ENZYME entry: EC

Accepted Name
multisite-specific tRNA:(cytosine-C(5))-methyltransferase
Reaction catalysed
  • cytidine(34) in tRNA precursor + S-adenosyl-L-methionine <=> 5-methylcytidine(34) in tRNA precursor + H(+) + S-adenosyl-L-homocysteine
  • cytidine(40) in tRNA precursor + S-adenosyl-L-methionine <=> 5-methylcytidine(40) in tRNA precursor + H(+) + S-adenosyl-L-homocysteine
  • cytidine(48) in tRNA + S-adenosyl-L-methionine <=> 5-methylcytidine(48) in tRNA + H(+) + S-adenosyl-L-homocysteine
  • cytidine(49) in tRNA + S-adenosyl-L-methionine <=> 5-methylcytidine(49) in tRNA + H(+) + S-adenosyl-L-homocysteine
  • The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
  • The incidence of modification depends on the cytosine position in tRNA.
  • At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNA(Leu)(CUA) and tRNA(Phe)(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine(48) or 5-methylcytosine(49), but never both in the same tRNA molecule.
  • The formation of 5-methylcytosine(34) and 5-methylcytosine(40) is a strictly intron-dependent process, whereas the formation of 5-methylcytosine(48) and 5-methylcytosine(49) is an intron- independent process.
  • Formerly EC
PRIAM enzyme-specific profiles2.1.1.202
KEGG Ligand Database for Enzyme Nomenclature2.1.1.202
IUBMB Enzyme Nomenclature2.1.1.202
MEDLINEFind literature relating to
Rhea expert-curated reactions2.1.1.202
P38205, NCL1_YEAST

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