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ENZYME

ENZYME entry: EC 2.1.1.207

Accepted Name
tRNA (cytidine(34)-2'-O)-methyltransferase
Alternative Name(s)
tRNA (cytidine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase
tRNA methyltransferase L
Reaction catalysed
  • cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine
  • 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine
Comment(s)
  • The enzyme from Escherichia coli catalyzes the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNA(Leu)CmAA and tRNA(Leu)cmnm(5)UmAA.
  • The enzyme is selective for the two tRNA(Leu) isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern.
  • Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N(6)-(isopentenyl)- 2-methylthioadenosine modification at position 37.
Cross-references
BRENDA2.1.1.207
EC2PDB2.1.1.207
ExplorEnz2.1.1.207
PRIAM enzyme-specific profiles2.1.1.207
KEGG Ligand Database for Enzyme Nomenclature2.1.1.207
IUBMB Enzyme Nomenclature2.1.1.207
IntEnz2.1.1.207
MEDLINEFind literature relating to 2.1.1.207
MetaCyc2.1.1.207
Rhea expert-curated reactions2.1.1.207
UniProtKB/Swiss-Prot
A1WBM9, TRML_ACISJA0KEQ6, TRML_AERHHD7A3L6, TRML_ANCN5
O31590, TRML_BACSUB2IBT9, TRML_BEII9A0KAS4, TRML_BURCH
Q0BBL1, TRML_BURCMA3NR15, TRML_BURP0P0AGJ8, TRML_ECOL6
P0AGJ7, TRML_ECOLIP32813, TRML_GEOSEP44868, TRML_HAEIN
Q042B4, TRML_LACGAA2RKW7, TRML_LACLMP69001, TRML_LISIN
Q721N0, TRML_LISMFC6X9I5, TRML_METGSP47588, TRML_MYCGE
P75257, TRML_MYCPNC7BSD3, TRML_PHOAAQ12E50, TRML_POLSJ
Q21LN7, TRML_SACD2B8CHC9, TRML_SHEPWQ48V41, TRML_STRPM
P74516, TRML_SYNY3Q74Y93, TRML_YERPE

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