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ENZYME entry: EC 2.1.1.207

Accepted Name
tRNA (cytidine(34)-2'-O)-methyltransferase.
Alternative Name(s)
tRNA (cytidine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase.
tRNA methyltransferase L.
Reaction catalysed
  • cytidine(34) in tRNA + S-adenosyl-L-methionine <=> 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine
  • 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine <=> 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine
Comment(s)
  • The enzyme from Escherichia coli catalyzes the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNA(Leu)CmAA and tRNA(Leu)cmnm(5)UmAA.
  • The enzyme is selective for the two tRNA(Leu) isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern.
  • Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N(6)-(isopentenyl)- 2-methylthioadenosine modification at position 37.
Cross-references
BRENDA2.1.1.207
EC2PDB2.1.1.207
ExplorEnz2.1.1.207
PRIAM enzyme-specific profiles2.1.1.207
KEGG Ligand Database for Enzyme Nomenclature2.1.1.207
IUBMB Enzyme Nomenclature2.1.1.207
IntEnz2.1.1.207
MEDLINEFind literature relating to 2.1.1.207
MetaCyc2.1.1.207
Rhea expert-curated reactions2.1.1.207
UniProtKB/Swiss-Prot
A1WBM9, TRML_ACISJ;  A0KEQ6, TRML_AERHH;  O31590, TRML_BACSU;  
B2IBT9, TRML_BEII9;  A0KAS4, TRML_BURCH;  Q0BBL1, TRML_BURCM;  
A3NR15, TRML_BURP0;  P0AGJ8, TRML_ECOL6;  P0AGJ7, TRML_ECOLI;  
P32813, TRML_GEOSE;  P44868, TRML_HAEIN;  Q042B4, TRML_LACGA;  
A2RKW7, TRML_LACLM;  P69001, TRML_LISIN;  Q721N0, TRML_LISMF;  
C6X9I5, TRML_METGS;  P47588, TRML_MYCGE;  P75257, TRML_MYCPN;  
C7BSD3, TRML_PHOAA;  Q12E50, TRML_POLSJ;  Q21LN7, TRML_SACD2;  
B8CHC9, TRML_SHEPW;  D7A3L6, TRML_STAND;  Q48V41, TRML_STRPM;  
P74516, TRML_SYNY3;  Q74Y93, TRML_YERPE;  

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