ENZYME entry: EC 2.1.1.224
| Accepted Name |
|---|
| 23S rRNA (adenine(2503)-C(8))-methyltransferase.
|
| Reaction catalysed |
|---|
| 2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin <=> S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
| Cofactor(s) |
|---|
| Iron-sulfur.
|
| Comment(s) |
|---|
- This enzyme is a member of the 'AdoMet radical' (radical SAM) family.
- S-adenosyl-L-methionine acts as both a radical generator and as the
source of the appended methyl group.
- Cfr is an plasmid-acquired methyltransferase that protects cells from
the action of antibiotics.
- The enzyme methylates adenosine at position 2503 of 23S rRNA by a
radical mechanism, transferring a CH(2) group from S-adenosyl-L-
methionine while retaining the hydrogen at the C-8 position of the
adenine.
- Cfr first transfers an CH(2) group to a conserved cysteine (Cys(338)
in Staphylococcus aureus), the generated radical from a second
S-adenosyl-L-methionine then attacks the methyl group, exctracting a
hydrogen.
- The formed radical forms a covalent intermediate with the adenine
group of the tRNA.
- The enzyme will also methylate 2-methyladenine produced by the action
of EC 2.1.1.192.
|
| Cross-references |
|---|
| BRENDA | 2.1.1.224 |
| EC2PDB | 2.1.1.224 |
| ExplorEnz | 2.1.1.224 |
| PRIAM enzyme-specific profiles | 2.1.1.224 |
| KEGG Ligand Database for Enzyme Nomenclature | 2.1.1.224 |
| IUBMB Enzyme Nomenclature | 2.1.1.224 |
| IntEnz | 2.1.1.224 |
| MEDLINE | Find literature relating to 2.1.1.224 |
| MetaCyc | 2.1.1.224 |
| Rhea expert-curated reactions | 2.1.1.224 |
| UniProtKB/Swiss-Prot |
| Q5WJ42, CFR_BACSK; | A7Z1T2, CFR_BACVZ; | A7FX96, CFR_CLOB1; |
| A5I5U3, CFR_CLOBH; | B1IL14, CFR_CLOBK; | A7GH77, CFR_CLOBL; |
| B1KZ37, CFR_CLOBM; | A9KK15, CFR_LACP7; | A5HBL2, CFR_STAAU; |
| Q9FBG4, CFR_STASC; | A2AXI2, CFR_STAWA; |
|
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