Expasy logo

ENZYME

ENZYME entry: EC 2.1.1.390

PURL: https://purl.expasy.org/enzyme/EC/2.1.1.390
Accepted Name
gentamicin X2 methyltransferase
Alternative Name(s)
gentamicin C-methyltransferase
Reaction catalysed
gentamicin X2 + AH2 + 2 S-adenosyl-L-methionine = geneticin + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
Comment(s)
  • The enzyme, isolated from the bacterium Micromonospora echinospora, has a single [4Fe-4S] cluster per monomer.
  • It is a radical S-adenosyl-L-methionine (SAM) enzyme with a methylcob(III)alamin cofactor.
  • The enzyme uses two molecues of SAM for the reaction.
  • One molecule forms a 5'-deoxyadenosyl radical, while the other is used to methylate the cobalamin cofactor.
  • It catalyzes methylation of the 6'-carbon of gentamicin X2 (GenX2) to produce genetricin (G418) during the biosynthesis of gentamicins.
  • The 6'-pro-R-hydrogen atom of GenX2 is stereoselectively abstracted by the 5'-deoxyadenosyl radical and methylation occurs with retention of configuration at C(6)'.
  • The regeneration of cob(I)alamin from cob(III)alamin is carried out with an as yet unidentified electron donor.
Cross-references
BRENDA2.1.1.390
EC2PDB2.1.1.390
ExplorEnz2.1.1.390
KEGG Ligand Database for Enzyme Nomenclature2.1.1.390
IUBMB Enzyme Nomenclature2.1.1.390
MEDLINEFind literature relating to 2.1.1.390
MetaCyc2.1.1.390
Rhea expert-curated reactions2.1.1.390

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.-.-.-