ID 2.1.1.398 DE isoflavone 3'-O-methyltransferase. CA a 3'-hydroxyisoflavone + S-adenosyl-L-methionine = a 3'-methoxyisoflavone CA + S-adenosyl-L-homocysteine + H(+). CC -!- The enzyme catalyzes a step in the biosynthesis of CC 3'-methoxypuerarin, an isoflavone with bioactive properties isolated CC from the roots of the leguminous plant Pueraria lobata var. CC leguminosa. CC -!- The highest activity has been found for 3'-hydroxydaidzein. The CC enzyme has low activity on the 3'-hydroxyflavones luteolin and CC quercetin. CC -!- 3'-hydroxypuerarin is not a substrate. //