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ENZYME entry: EC

Accepted Name
tRNA carboxymethyluridine synthase
Reaction catalysed
acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA <=> 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine
  • The enzyme, found in eukaryotes, most archaea, and some bacteria, catalyzes the first step in modification of the wobble uridine base of certain tRNAs.
  • In eukaryotes the enzyme is a complex of six conserved subunits, with ELP3 being the catalytic subunit.
  • In archaea and bacteria the enzyme consists of a single subunit, homologous to ELP3.
  • The enzyme contains an [4Fe-4S] cluster and uses radical chemistry. A 5'-deoxyadenosyl radical generated in the radical AdoMet (SAM) domain attacks the acetyl-CoA donor, activating its methyl group, which forms a C-C bond with C5 of the uridine moiety.
PRIAM enzyme-specific profiles2.3.1.311
KEGG Ligand Database for Enzyme Nomenclature2.3.1.311
IUBMB Enzyme Nomenclature2.3.1.311
MEDLINEFind literature relating to
Rhea expert-curated reactions2.3.1.311

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