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ENZYME

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ENZYME entry: EC 2.3.1.311

Accepted Name
tRNA carboxymethyluridine synthase
Reaction catalysed
acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine
Comment(s)
  • The enzyme, found in eukaryotes, most archaea, and some bacteria, catalyzes the first step in modification of the wobble uridine base of certain tRNAs.
  • In eukaryotes the enzyme is a complex of six conserved subunits, with ELP3 being the catalytic subunit.
  • In archaea and bacteria the enzyme consists of a single subunit, homologous to ELP3.
  • The enzyme contains an [4Fe-4S] cluster and uses radical chemistry. A 5'-deoxyadenosyl radical generated in the radical AdoMet (SAM) domain attacks the acetyl-CoA donor, activating its methyl group, which forms a C-C bond with C5 of the uridine moiety.
Cross-references
BRENDA2.3.1.311
EC2PDB2.3.1.311
ExplorEnz2.3.1.311
PRIAM enzyme-specific profiles2.3.1.311
KEGG Ligand Database for Enzyme Nomenclature2.3.1.311
IUBMB Enzyme Nomenclature2.3.1.311
IntEnz2.3.1.311
MEDLINEFind literature relating to 2.3.1.311
MetaCyc2.3.1.311
Rhea expert-curated reactions2.3.1.311
UniProtKB/Swiss-Prot
Q93ZR1, ELP3_ARATHQ2KJ61, ELP3_BOVINQ60LW7, ELP3_CAEBR
Q23651, ELP3_CAEELQ5ZHS1, ELP3_CHICKQ5RIC0, ELP3_DANRE
A0A1C7D1B7, ELP3_DEHMCQ1ZXC6, ELP3_DICDIQ9VQZ6, ELP3_DROME
Q9H9T3, ELP3_HUMAND5VRB9, ELP3_METIMQ58536, ELP3_METJA
Q9CZX0, ELP3_MOUSEQ7X7L3, ELP3_ORYSJO14023, ELP3_SCHPO
Q5HZM6, ELP3_XENLAQ6NVL5, ELP3_XENTRQ02908, ELP3_YEAST

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