ID 2.4.1.266 DE glucosyl-3-phosphoglycerate synthase. AN glucosylphosphoglycerate synthase. AN GPG synthase. CA (2R)-3-phosphoglycerate + an NDP-alpha-D-glucose = (2R)-2-O-(alpha-D- CA glucopyranosyl)-3-phospho-glycerate + a ribonucleoside 5'-diphosphate + CA H(+). CC -!- The enzyme is involved in biosynthesis of 2-O-(alpha-D- CC glucopyranosyl)-D-glycerate via the two-step pathway in which CC glucosyl-3-phosphoglycerate synthase catalyzes the conversion of GDP- CC glucose and 3-phospho-D-glycerate into 2-O-(alpha-D-glucopyranosyl)- CC 3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D- CC glucopyranosyl)-D-glycerate by EC 3.1.3.85. CC -!- The activity is dependent on divalent cations (Mn(2+), Co(2+), CC or Mg(2+)). CC -!- The enzyme from Persephonella marina shows moderate flexibility on CC the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP- CC glucose, GDP-glucose) but is strictly specific for glucose. CC -!- The enzyme is also strictly specific for 3-phospho-D-glycerate as CC acceptor. CC -!- The enzyme from Methanococcoides burtonii is strictly specific for CC GDP-glucose and 3-phospho-D-glycerate. CC -!- This enzyme catalyzes the first glucosylation step in methylglucose CC lipopolysaccharide biosynthesis in mycobacteria. DR Q12XX4, GPGS_METBU ; Q7U0E1, GPGS_MYCBO ; Q73WU1, GPGS_MYCPA ; DR A0R2E6, GPGS_MYCS2 ; P9WMW8, GPGS_MYCTO ; P9WMW9, GPGS_MYCTU ; DR C0QRQ2, GPGS_PERMH ; A9BHI9, GPGS_PETMO ; //