ID 2.4.1.385 DE sterol 27-beta-glucosyltransferase. CA a 27-hydroxysterol + UDP-alpha-D-glucose = a sterol 27-beta-D-glucoside + CA H(+) + UDP. CC -!- The enzyme, isolated from the plant Withania somnifera (ashwagandha), CC transfers D-glucose to a beta-hydroxyl group present at the C-27 CC position in sterols/withanolides, provided the substrate possesses a CC 17alpha-OH group. CC -!- Natural substrates are 17alpha-hydroxywithaferin A, 27beta- CC hydroxywithanone, and 5alpha,6beta,17alpha,27beta- CC tetrahydroxywithanolide. CC -!- The enzyme's activity with withanolide A and withanolide U, which CC lack a 17alpha-hydroxyl group, suggests it may also be able to CC glucosylate the C-20 beta-OH position, although this has not been CC verified yet. CC -!- The enzyme does not glucosylate sterols at the C-3 position. //