ID 2.4.99.18 DE dolichyl-diphosphooligosaccharide--protein glycotransferase. CA a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl- CA [protein] = a di-trans,poly-cis-dolichyl diphosphate + H(+) + N(4)- CA (oligosaccharide-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl- CA beta-D-glucosaminyl)-L-asparaginy-[protein]. CC -!- Occurs in eukaryotes that form a glycoprotein by the transfer of a CC glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an CC L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- CC (Xaa not Pro) in nascent polypeptide chains. CC -!- The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2. CC -!- However, smaller oligosaccharides derived from it and CC oligosaccharides with additional monosaccharide units attached may be CC involved. CC -!- Man3GlcNAc2 seems to be common for all of the oligosaccharides CC involved with the terminal N-acetylglucosamine linked to the protein CC L-asparagine. CC -!- Occurs on the cytosolic face of the endoplasmic reticulum. CC -!- The dolichol involved normally has 14-21 isoprenoid units with two CC trans double-bonds at the omega end, and the rest of the double-bonds CC in cis form. CC -!- Formerly EC 2.4.1.119. DR Q93ZY3, STT3A_ARATH; Q2KJI2, STT3A_BOVIN; F1PJP5, STT3A_CANLF; DR P46977, STT3A_HUMAN; P46978, STT3A_MOUSE; Q6F2Z1, STT3A_ORYSJ; DR Q5RCE2, STT3A_PONAB; Q9FX21, STT3B_ARATH; E2RG47, STT3B_CANLF; DR Q8TCJ2, STT3B_HUMAN; Q3TDQ1, STT3B_MOUSE; Q7XQ88, STT3B_ORYSJ; DR P46975, STT3_CAEEL ; Q54NM9, STT3_DICDI ; O94335, STT3_SCHPO ; DR P39007, STT3_YEAST ; //