ID 2.7.1.163 DE hygromycin B 4-O-kinase. AN hygromycin-B kinase. CA ATP + hygromycin B = 4-O-phosphohygromycin B + ADP + H(+). CC -!- Phosphorylates the antibiotic hygromycin B. CC -!- Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) CC catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, CC found in Escherichia coli carrying a plasmid conferring resistance to CC hygromycin-B, forms 4-O-phosphohygromycin B. CC -!- Formerly EC 2.7.1.n6. DR P00557, KHYB_ECOLX ; //