ID 2.7.1.190 DE aminoglycoside 2''-phosphotransferase. AN aminoglycoside (2'') kinase. AN APH(2''). AN gentamicin kinase. AN gentamicin phosphotransferase. CA a gentamycin + GTP = a gentamycin 2''-phosphate + GDP + H(+). CC -!- This bacterial enzyme phosphorylates many 4,6-disubstituted CC aminoglycoside antibiotics that have a hydroxyl group at position CC 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, CC arbekacin, amikacin, gentamicin C, sisomicin and netilmicin. CC -!- In most, but not all, cases the phosphorylation confers resistance CC against the antibiotic. CC -!- Some forms of the enzyme use ATP as a phosphate donor in appreciable CC amount. CC -!- The enzyme is often found as a bifunctional enzyme that also CC catalyzes 6'-aminoglycoside N-acetyltransferase activity. CC -!- The bifunctional enzyme is the most clinically important CC aminoglycoside-modifying enzyme in Gram-positive bacteria, CC responsible for high-level resistance in both Enterococci and CC Staphylococci. DR P0A0C2, AACA_ENTFA ; Q52S23, AACA_ENTFC ; Q7ATH9, AACA_MAMSC ; DR P0A0C0, AACA_STAAM ; P0A0C1, AACA_STAAU ; Q5HMP3, AACA_STAEQ ; DR Q4L605, AACA_STAHJ ; Q7ATH7, AACA_STAWA ; //