ID 2.7.7.73 DE sulfur carrier protein ThiS adenylyltransferase. CA [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + ATP + H(+) = [sulfur- CA carrier protein ThiS]-C-terminal Gly-Gly-AMP + diphosphate. CC -!- The enzyme catalyzes the adenylation of ThiS, a sulfur carrier CC protein involved in the biosynthesis of thiamine. CC -!- The enzyme shows significant structural similarity to ubiquitin- CC activating enzyme. CC -!- In Escherichia coli, but not in Bacillus subtilis, the enzyme forms a CC cross link from Cys-184 to the ThiS carboxy terminus (the position CC that is also thiolated) via an acyldisulfide. CC -!- Formerly EC 2.7.7.n3. DR O31619, THIF_BACSU ; P30138, THIF_ECOLI ; Q72J02, TTUC_THET2 ; //