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ENZYME

ENZYME entry: EC 2.8.4.5

Accepted Name
tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
Alternative Name(s)
methylthio-threonylcarbamoyl-adenosine transferase B
Reaction catalysed
N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
Comment(s)
  • The enzyme, which is a member of the S-adenosyl-L-methionine- dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur.
  • The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover.
  • The identity of the electron donor is not known.
Cross-references
BRENDA2.8.4.5
EC2PDB2.8.4.5
ExplorEnz2.8.4.5
KEGG Ligand Database for Enzyme Nomenclature2.8.4.5
IUBMB Enzyme Nomenclature2.8.4.5
MEDLINEFind literature relating to 2.8.4.5
MetaCyc2.8.4.5
Rhea expert-curated reactions2.8.4.5
UniProtKB/Swiss-Prot
O29021, AMTAB_ARCFUQ58277, AMTAB_METJAO26914, AMTAB_METTH
Q9UXX9, AMTAB_PYRABO59545, AMTAB_PYRHOQ8MXQ7, CDKAL_CAEEL
Q6PG34, CDKAL_DANREQ7K4W1, CDKAL_DROMEQ291H5, CDKAL_DROPS
Q5VV42, CDKAL_HUMANQ91WE6, CDKAL_MOUSEQ6NS26, CDKAL_XENLA
Q6P4Y0, CDKAL_XENTRO66772, MTAB_AQUAEP54462, MTAB_BACSU
Q9Z874, MTAB_CHLPNQ49573, MTAB_MALIOQ9ZDB6, MTAB_RICPR
Q9WZT7, MTAB_THEMAO83293, MTAB_TREPA

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