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ENZYME entry: EC 2.8.4.5

Accepted Name
tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase.
Alternative Name(s)
methylthio-threonylcarbamoyl-adenosine transferase B.
Reaction catalysed
[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine <=> 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine
Comment(s)
  • The enzyme, which is a member of the S-adenosyl-L-methionine- dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur.
  • The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover.
  • The identity of the electron donor is not known.
Cross-references
BRENDA2.8.4.5
EC2PDB2.8.4.5
ExplorEnz2.8.4.5
PRIAM enzyme-specific profiles2.8.4.5
KEGG Ligand Database for Enzyme Nomenclature2.8.4.5
IUBMB Enzyme Nomenclature2.8.4.5
IntEnz2.8.4.5
MEDLINEFind literature relating to 2.8.4.5
MetaCyc2.8.4.5
Rhea expert-curated reactions2.8.4.5
UniProtKB/Swiss-Prot
O29021, AMTAB_ARCFU;  Q58277, AMTAB_METJA;  O26914, AMTAB_METTH;  
Q9UXX9, AMTAB_PYRAB;  O59545, AMTAB_PYRHO;  Q8MXQ7, CDKAL_CAEEL;  
Q6PG34, CDKAL_DANRE;  Q7K4W1, CDKAL_DROME;  Q291H5, CDKAL_DROPS;  
Q5VV42, CDKAL_HUMAN;  Q91WE6, CDKAL_MOUSE;  Q6NS26, CDKAL_XENLA;  
Q6P4Y0, CDKAL_XENTR;  O66772, MTAB_AQUAE;  P54462, MTAB_BACSU;  
Q9Z874, MTAB_CHLPN;  Q49573, MTAB_MALIO;  Q9ZDB6, MTAB_RICPR;  
Q9WZT7, MTAB_THEMA;  O83293, MTAB_TREPA;  

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