ID   3.1.1.109
DE   iron(III) salmochelin esterase.
CA   (1) Fe(III)-di(C-5-deoxy-beta-D-glucosyl)-enterobactin + H2O = Fe(III)-
CA   {N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine]2} + H(+).
CA   (2) Fe(III)-C-5-deoxy-beta-D-glucosyl-enterobactin + H2O = Fe(III)-
CA   {di[N-(2,3-dihydroxybenzoyl)-L-seryl]-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine} + H(+).
CA   (3) Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-
CA   glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2} + H(+) + H2O = Fe(III)-
CA   [N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2 +
CA   N-(2,3-dihydroxybenzoyl)-L-serine.
CA   (4) Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-
CA   glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2} + H(+) + H2O = Fe(III)-
CA   {N-(2,3-dihydroxybenzoyl)-L-seryl-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine} + N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine.
CA   (5) Fe(III)-{di[N-(2,3-dihydroxybenzoyl)-L-seryl]-N-(C-5-[deoxy-beta-D-
CA   glucosyl]-2,3-dihydroxybenzoyl)-L-serine} + H(+) + H2O = Fe(III)-{N-(2,3-
CA   dihydroxybenzoyl)-L-seryl-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine} + N-(2,3-dihydroxybenzoyl)-L-serine.
CA   (6) Fe(III)-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-
CA   serine]2 + H(+) + H2O = Fe(III)-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine] + N-(C-5-[deoxy-beta-D-glucosyl]-2,3-
CA   dihydroxybenzoyl)-L-serine.
CC   -!- This bacterial enzyme is present in pathogenic Salmonella species,
CC       uropathogenic and avian pathogenic Escherichia coli strains,
CC       and certain Klebsiella strains.
CC   -!- The enzyme acts on iron(III)-bound enterobactin and C-glucosylated
CC       derivatives known as salmochelins.
CC   -!- Unlike EC 3.1.1.107, (IroE), IroD prefers iron(III)-bound
CC       siderophores as substrates, and is assumed to act after the iron-
CC       siderophore complexes are imported into the cell.
CC   -!- It catalyzes several hydrolytic reactions, producing a mixture of
CC       iron(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] complex and salmochelin
CC       SX.
DR   A0A0H2V660, IROD_ECOL6 ;
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