ID 3.1.21.5 DE type III site-specific deoxyribonuclease. AN type III restriction enzyme. CA Endonucleolytic cleavage of DNA to give specific double-stranded CA fragments with terminal 5'-phosphates. CC -!- Has an absolute requirement for ATP, but does not hydrolyze it; CC S-adenosyl-L-methionine stimulates the reaction, but is not CC absolutely required. CC -!- Recognize specific short DNA sequences and cleave a short distance CC away from the recognition sequence. CC -!- Exist as complexes with enzymes of similar specificity listed under CC EC 2.1.1.72 or EC 2.1.1.73. CC -!- See the REBASE database for a complete list of these enzymes: CC http://rebase.neb.com/rebase/ CC -!- Formerly EC 3.1.24.3 and EC 3.1.24.4. DR P25241, T3RE_BACC1 ; P08764, T3RE_BPP1 ; Q5ZND2, T3RE_ECOLX ; DR P40815, T3RE_SALTY ; P44105, T3RH_HAEIN ; //