ID 3.1.4.50 DE glycosylphosphatidylinositol phospholipase D. AN glycoprotein phospholipase D. AN GPI-PLD. AN phosphatidylinositol-glycan-specific phospholipase D. AN phosphatidylinositol phospholipase D. AN phosphatidylinositol-specific phospholipase D. CA an alpha-D-GlcN-(1->6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol CA + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + a 1,2-diacyl- CA sn-glycero-3-phosphate + H(+). CC -!- This enzyme is also active when O-4 of the glucosamine is substituted CC by carrying the oligosaccharide that can link a protein to the CC structure. CC -!- It therefore cleaves proteins from the lipid part of the CC glycosylphosphatidylinositol (GPI) anchors, but does so by CC hydrolysis, whereas EC 4.6.1.14 does so by elimination. CC -!- It acts on plasma membranes only after solubilization of the CC substrate with detergents or solvents, but it may act on CC intracellular membranes. DR P80109, PHLD_BOVIN ; P80108, PHLD_HUMAN ; O70362, PHLD_MOUSE ; DR Q8R2H5, PHLD_RAT ; Q86AV9, PLDG_DICDI ; //