ID 3.2.1.159 DE alpha-neoagaro-oligosaccharide hydrolase. AN alpha-NAOS hydrolase. AN alpha-neoagarooligosaccharide hydrolase. CA Hydrolysis of the 1,3-alpha-L-galactosidic linkages of neoagaro- CA oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L- CA galactose and D-galactose. CC -!- When neoagarohexaose is used as a substrate, the oligosaccharide is CC cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose CC and agaropentaose, which is further hydrolyzed to agarobiose and CC agarotriose. CC -!- With neoagarotetraose as substrate, the products are predominantly CC agarotriose and 3,6-anhydro-L-galactose. CC -!- In Vibrio sp. the actions of EC 3.2.1.81 and EC 3.2.1.159 can be used CC to degrade agarose to 3,6-anhydro-L-galactose and D-galactose. //