ID 3.2.1.214 DE exo beta-1,2-glucooligosaccharide sophorohydrolase (non-reducing end). CA [(1->2)-beta-D-glucosyl](n) + H2O = [(1->2)-beta-D-glucosyl](n-2) + CA sophorose. CC -!- The enzyme, characterized from the bacterium Parabacteroides CC distasonis, specifically hydrolyzes (1->2)-beta-D- CC glucooligosaccharides to sophorose. CC -!- The best substrates are the tetra- and pentasaccharides. CC -!- The enzyme is not able to cleave the trisaccharide, and activity with CC longer linear (1->2)-beta-D-glucans is quite low. CC -!- This enzyme acts in exo mode and is not able to hydrolyze cyclic CC (1->2)-beta-D-glucans. DR A6LGF6, SOPH_PARD8 ; //