ID 3.2.1.217 DE exo-acting protein-alpha-N-acetylgalactosaminidase. CA (1) 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + H2O = CA L-seryl-[protein] + N-acetyl-D-galactosamine. CA (2) 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + H2O = CA L-threonyl-[protein] + N-acetyl-D-galactosamine. CC -!- The enzyme, which belongs to the glycosylhydrolase 31 (GH31) family, CC is an exo-acting alpha-N-acetylgalactosaminidase that acts on the CC innermost alpha-GalNAc residues at the core of O-glycans when no CC other saccharides are attached to it. CC -!- Unlike EC 3.2.1.49, it is not able to act on blood group A antigen. //