ID   3.2.1.220
DE   ipecoside beta-D-glucosidase.
AN   6-O-methyl-deacetylisoipecoside beta-glucosidase.
AN   IpeGlu(1).
CA   (1) H2O + ipecoside = D-glucose + ipecoside aglycone.
CA   (2) H2O + N-deacetylipecoside = D-glucose + N-deacetylipecoside aglycone.
CA   (3) 6-O-methyl-N-deacetylipecoside + H2O = 6-O-methyl-N-deacetylipecoside
CA   aglycone + D-glucose.
CC   -!- The enzyme, isolated from the roots of the plant Carapichea
CC       ipecacuanha, preferentially hydrolyzes glucosidic ipecoside alkaloids
CC       except for their lactams, but shows poor or no activity toward other
CC       substrates.
CC   -!- IpeGlu(1) activity is extremely poor toward 7-O-methyl and 6,7-
CC       O,O-dimethyl derivatives. However, 6-O-methyl derivatives are
CC       hydrolyzed as efficiently as non-methylated substrates.
CC   -!- IpeGlu(1) accepts both 1alpha(S)-N-deacetylisoipecoside and 1beta(R)-
CC       N-deacetylipecoside epimers as substrate, with preference for the
CC       1beta(R)-epimer.
CC   -!- 6-O-methyl-N-deacetylisoipecoside is an intermediate in the
CC       biosynthesis of the medicinal alkaloid emetine.
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