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ENZYME

ENZYME entry: EC 3.2.1.97

Accepted Name
endo-alpha-N-acetylgalactosaminidase
Alternative Name(s)
D-galactosyl-3-(N-acetyl-alpha-D-galactosaminyl)-L-serine mucinaminohydrolase
D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl- galactosaminohydrolase
endo-alpha-acetylgalactosaminidase
endo-alpha-GalNAc-ase
endo-alpha-N-acetyl-D-galactosaminidase
glycopeptide alpha-N-acetylgalactosaminidase
mucinaminylserine mucinaminidase
Reaction catalysed
  • H2O + O(3)-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] <=> beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine + L-seryl-[protein]
  • H2O + O(3)-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] <=> beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine + L-threonyl-[protein]
Comment(s)
  • The enzyme catalyzes the liberation of Gal-(1->3)-beta-GalNAc alpha- linked to serine or threonine residues of mucin-type glycoproteins.
  • EngBF from Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc.
  • The enzymes from Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. release of the core 2 trisaccharide Gal-(1->3)- beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc- (1->3)-beta-GalNAc).
  • The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
  • Formerly EC 3.2.1.110.
Cross-references
BRENDA3.2.1.97
EC2PDB3.2.1.97
ExplorEnz3.2.1.97
PRIAM enzyme-specific profiles3.2.1.97
KEGG Ligand Database for Enzyme Nomenclature3.2.1.97
IUBMB Enzyme Nomenclature3.2.1.97
IntEnz3.2.1.97
MEDLINEFind literature relating to 3.2.1.97
MetaCyc3.2.1.97
Rhea expert-curated reactions3.2.1.97
UniProtKB/Swiss-Prot
A9WNA0, GH101_RENSMQ2MGH6, GH101_STRPNQ8DR60, GH101_STRR6

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.2.1.-
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