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ENZYME

ENZYME entry: EC 3.4.22.40

Accepted Name
bleomycin hydrolase
Alternative Name(s)
aminopeptidase C (Lactococcus lactis)
Reaction catalysed
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
Comment(s)
  • The active sites are on the walls of a central channel through the molecule, and access of substrate molecules to them is obstructed by this and by the C-terminus of each polypeptide chain.
  • Bleomycin can scarcely be the natural substrate, and there are reports of limited endopeptidase activity.
  • Known from bacteria as well as eukaryotic organisms.
  • Hydrolase H from chicken muscle has many similarities to bleomycin hydrolase, but hydrolyzes Ph-CO-Arg-2-naphthylamine as well as aminopeptidase substrates.
  • Belongs to peptidase family C1.
Cross-references
BRENDA3.4.22.40
EC2PDB3.4.22.40
ExplorEnz3.4.22.40
PRIAM enzyme-specific profiles3.4.22.40
KEGG Ligand Database for Enzyme Nomenclature3.4.22.40
IUBMB Enzyme Nomenclature3.4.22.40
IntEnz3.4.22.40
MEDLINEFind literature relating to 3.4.22.40
MetaCyc3.4.22.40
Rhea expert-curated reactions3.4.22.40
UniProtKB/Swiss-Prot
B3LP78, BLH1_YEAS1C7GPC1, BLH1_YEAS2B5VQH0, BLH1_YEAS6
A6ZRK4, BLH1_YEAS7C8ZFZ7, BLH1_YEAS8Q01532, BLH1_YEAST
P87362, BLMH_CHICKQ13867, BLMH_HUMANQ8R016, BLMH_MOUSE
P13019, BLMH_RABITP70645, BLMH_RATQ48543, PEPC_LACDL
Q10744, PEPC_LACHEQ9CEG3, PEPC_LACLAQ04723, PEPC_LACLC
Q928V0, PEPC_LISINO69192, PEPC_LISMOQ56115, PEPC_STRTR

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