ID   3.4.24.3
DE   microbial collagenase.
AN   clostridiopeptidase A.
AN   Clostridium histolyticum collagenase.
AN   collagenase A.
AN   collagenase I.
CA   Digestion of native collagen in the triple helical region at Xaa-|-Gly
CA   bonds. With synthetic peptides, a preference is shown for Gly at P3 and
CA   P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.
CC   -!- Six species of metalloendopeptidase acting on native collagen can be
CC       isolated from the medium of Clostridium histolyticum.
CC   -!- Class I has forms alpha (68 kDa), beta (115 kDa) and gamma (79 kDa);
CC       class II has delta (100 kDa), epsilon (110 kDa) and zeta (125 kDa).
CC   -!- The two classes are immunologically crossreactive, but have
CC       significantly different sequences, and different specificities such
CC       that their actions on collagen are complementary.
CC   -!- The enzymes also act as peptidyl-tripeptidases.
CC   -!- Variants of the enzyme have been purified from Bacillus cereus,
CC       Empedobacter collagenolyticum, Pseudomonas marinoglutinosa,
CC       and species of Vibrio, Vibrio B-30 (ATCC 21250) and V.alginolyticus
CC       (previously Achromobacter iophagus).
CC   -!- Also known from Streptomyces sp.
CC   -!- Belongs to peptidase family M9.
CC   -!- Formerly EC 3.4.4.19, EC 3.4.24.8 and EC 3.4.99.5.
DR   Q81BJ6, COLA_BACCR ;  P43153, COLA_CLOPE ;  P43154, COLA_VIBAL ;
DR   Q9KRJ0, COLA_VIBCH ;  Q56696, COLA_VIBPA ;  Q8D4Y9, COLA_VIBVU ;
DR   Q9X721, COLG_HATHI ;  Q46085, COLH_HATHI ;  B9J3S4, COLQ1_BACCQ;
DR   Q899Y1, COLT_CLOTE ;
//