ENZYME entry: EC 3.5.1.97

Accepted Name

acyl-homoserine-lactone acylase

Alternative Name(s)

acyl-homoserine lactone acylase

AHL-acylase

N-acyl-homoserine lactone acylase

quorum-quenching AHL acylase

quorum-quenching enzyme

Reaction catalysed

an N-acyl-L-homoserine lactone + H2O = L-homoserine lactone + a carboxylate

Comment(s)

Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing.
Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signaling which, in turn, initiates the expression of particular virulence genes.
Plants or animals capable of degrading AHLs would have a therapeutic advantage in avoiding bacterial infection as they could prevent AHL- signaling and the expression of virulence genes in quorum-sensing bacteria.
This quorum-quenching enzyme removes the fatty-acid side chain from the homoserine lactone ring of AHL-dependent quorum-sensing signal molecules.
It has broad specificity for AHLs with side changes ranging in length from 11 to 14 carbons.
Substituents at the 3'-position, as found in N-(3-oxododecanoyl)-L- homoserine lactone, do not affect this activity.

Cross-references

BRENDA

EC2PDB

ExplorEnz

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-