ID 4.1.1.88 DE biotin-independent malonate decarboxylase. AN malonate carboxy-lyase (biotin-independent). AN malonate decarboxylase. AN MDC. CA H(+) + malonate = acetate + CO2. CC -!- Two types of malonate decarboxylase are currently known, both of CC which form multienzyme complexes. CC -!- This enzyme is a cytosolic protein that is biotin-independent. CC -!- The other type is a biotin-dependent, Na(+)-translocating enzyme that CC includes both soluble and membrane-bound components (cf. EC 7.2.4.4). CC -!- As free malonate is chemically rather inert, it has to be activated CC prior to decarboxylation. CC -!- In both enzymes, this is achieved by exchanging malonate with an CC acetyl group bound to an acyl-carrier protiein (ACP), to form CC malonyl-ACP and acetate, with subsequent decarboxylation regenerating CC the acetyl-ACP. CC -!- The ACP subunit of both enzymes differs from that found in fatty-acid CC biosynthesis by having phosphopantethine attached to a serine side- CC chain as 2'-(5-triphosphoribosyl)-3'-dephospho-CoA rather than as CC phosphopantetheine 4'-phosphate. CC -!- The individual enzymes involved in carrying out the reaction of this CC enzyme complex are EC 2.3.1.187, EC 2.3.1.39 and EC 4.1.1.87. CC -!- The carboxy group is lost with retention of configuration. //