ID   4.1.99.25
DE   L-tryptophan isonitrile synthase.
CA   D-ribulose 5-phosphate + L-tryptophan = (2S)-3-(1H-indol-3-yl)-2-
CA   isocyanopropanoate + formaldehyde + H(+) + H2O + hydroxyacetone +
CA   phosphate.
CC   -!- The enzymes from cyanobacteria that belong to the Stigonematales
CC       order participate in the biosynthesis of hapalindole-type alkaloids.
CC   -!- According to the proposed mechanism, the enzyme forms an imine
CC       intermediate composed of linked L-tryptophan and D-ribulose
CC       5-phosphate, followed by loss of the phosphate group and formation of
CC       a beta-keto imine and keto-enol tautomerization.
CC   -!- This is followed by a C-C bond cleavage, the release of acetol, and a
CC       retro aldol type reaction that releases formaldehyde and forms the
CC       final product. cf. EC 4.1.99.24.
DR   V5TF65, AMBI1_FISAU;  V5TES5, AMBI2_FISAU;
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