ID 4.2.1.111 DE 1,5-anhydro-D-fructose dehydratase. AN 1,5-anhydro-D-arabino-hex-2-ulose dehydratase. AN 1,5-anhydro-D-fructose 4-dehydratase. AN 1,5-anhydro-D-fructose hydrolyase. AN AF dehydratase. AN AFDH. CA 1,5-anhydro-D-fructose = ascopyrone M + H2O. CC -!- Catalyzes one of the steps in the anhydrofructose pathway, which CC leads to the degradation of glycogen and starch via 1,5-anhydro-D- CC fructose. CC -!- The other enzymes involved in this pathway are EC 4.2.1.110, CC EC 4.2.2.13 and EC 5.3.2.7. CC -!- Unlike EC 4.2.1.110 the enzyme is specific for 1,5-anhydro-D-fructose CC as substrate and shows no activity toward aldose-2-uloses such as CC 2-dehydroglucose; in addition, it is inhibited by its end-product CC ascopyrone M and it cannot convert the end-product ascopyrone M into CC microthecin, as can EC 4.2.1.110. //